GrainGenes Marker Report: Xwmc168-7A
Locus
Xwmc168-7A
Type
Microsatellite
Chromosome
7A
Chromosome Arm
7AS
Map
Kofa/UC1113-7A 28.6 Ta-SSR-2004-7A 26 Ta-Synthetic/Opata-GPW-7A 28.5 Ta-Synthetic/Opata-SSR2-7A 17.3
[ Show Nearby Loci ]
Map Data
Durum wheat, Kofa x UC1113 Wheat, Consensus SSR, 2004 Wheat, Synthetic x Opata, GPW Wheat, Synthetic x Opata, SSR 2
Probe
WMC168
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc168 wmc168
Probe for Locus
WMC168
Locus
Xwmc168-7A Xwmc168 wmc168
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 9d2 Donor: Bernard, Clermont-Ferrand Trimmed to Rsa1 site at 5' end, and to insert at 3' end. (CT)27 338 to 391. Big difference between Tms of primers.
Type
PCR SSR
PCR primers
Forward AAcAcAAAAgATccAAcgAcAc at 91 length 22 bases, Tm 63
Reverse cAgTATAgAAggATTTTgAgAg at 388 length 22 bases, Tm 52
SSR size
319 bases expected, Chinese Spring
Amplification Conditions
51C anneal, polymorphic product on agarose; 51C anneal, polymorphic on acrylamide.;
Sequence
WMC168
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc168-7A
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