GrainGenes Marker Report: Xwmc175-2B
Locus
Xwmc175-2B
Type
Microsatellite
Chromosome
2B
Chromosome Arm
2BL
Map
Chinese_Spring_Deletion_SSR_2B 0.695
[ Show all 6 ]
[ Show Nearby Loci ]
Breakpoint Interval
2BL4-0.50-0.89
Map Data
Durum wheat, Svevo x Ciccio
[ Show all 7 ]
Probe
WMC175
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc175
[ Show all 12 ]
Probe for Locus
WMC175
Locus
Xwmc175-2B
[ Show all 13 ]
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 9b4 Donor: Bernard, Clermont-Ferrand Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 135 bases located on 2B, 145 bases located on 2B, 181 bases located on 2B, 256 bases located on 2B Trimmed to insert at 5' end and to Rsa1 at 3' end. (CA)16 138 to 169.
Type
PCR SSR
PCR primers
Forward gcTcAgTcAAAccgcTAcTTcT at 17 length 22 bases, Tm 62
Reverse cAcTAcTccAATcTATcgccgT at 248 length 22 bases, Tm 62
SSR size
253 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product;
Sequence
WMC175
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc175-2B
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