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GrainGenes Marker Report: Xwmc177

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Locus	Xwmc177
Type	Microsatellite
Chromosome	2A
Map	Ta-NxW-2A 83.9 Wheat, UC1110 x PI610750 2A 50 Wheat-Composite2004-2A 22
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004 Wheat, Nanda2419 x Wangshuibai Wheat, UC1110 x PI610750
Probe	WMC177
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	Xwmc177-2A wmc177-2A wmc177
Probe for Locus	WMC177
Locus	Xwmc177-2A Xwmc177 wmc177-2A wmc177
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 9h4 Donor: Bernard, Clermont-Ferrand Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 190 bases located on 2A Trimmed to insert at 5' end and to Rsa1 at 3' end. After first 40 bases is similar to 7c7(CA)21 37 to 78, (CGCA)10. Bases 81 to 101 show homology to 17e7 bases 273 to 293 (21 in longest run, maxScore = 21). Bases 37 to 70 show homology to 10h2 bases 68 to 101 (34 in longest run, maxScore = 26).
Type	PCR SSR
PCR primers	Forward AgggcTcTcTTTAATTcTTgcT at 13 length 22 bases, Tm 59 Reverse ggTcTATcgTAATccAccTgTA at 175 length 22 bases, Tm 57
SSR size	184 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC177
Source Species	Triticum aestivum