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GrainGenes Marker Report: Xwmc182-7B

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Locus	Xwmc182-7B
Type	Microsatellite
Chromosome	7B
Map	Kofa/UC1113-7B 38.7 Louise/Penawawa-7B 57.6 Ta-SSR-2004-7B 51.9
	[ Show Nearby Loci ]
Map Data	Durum wheat, Kofa x UC1113 Wheat, Consensus SSR, 2004 Wheat, Louise x Penawawa
Probe	WMC182
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs	wmc182 [ Show all 18 ]
Probe for Locus	WMC182
Locus	Xwmc182-7A [ Show all 19 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 9g6 Donor: Bernard, Clermont-Ferrand Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 165 bases located on 7B Trimmed to Rsa1 at both ends. (CT)16 194 to 225.
Type	PCR SSR
PCR primers	Forward gTATcTcAcgAgcATAAcAcAA at 91 length 22 bases, Tm 57 Reverse gAAAgTgTATggATcATTAggc at 228 length 22 bases, Tm 57
SSR size	159 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC182
Source Species	Triticum aestivum