Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Marker Report: Xwmc195-7B

[Submit comment/correction]

Locus	Xwmc195-7B
Type	Microsatellite
Map	Kofa/UC1113-7B 124.5
	[ Show Nearby Loci ]
Map Data	Durum wheat, Kofa x UC1113
Probe	WMC195
Possible Orthologs	wmc195 wmc195-7B
Probe for Locus	WMC195
Locus	Xwmc195-7B wmc195 wmc195-7B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 4c10 Donor: Morell, Canberra Trimmed to insert at both ends (removing mostly Ns). Small microsatellite. (CCG)5 82-96, (CCG)4 108-119.
Type	PCR SSR
PCR primers	Forward AgAgTTcAgATcTAcAAggcgg at 16 length 22 bases, Tm 62 Reverse gggccccAAATTAATTAgcAcc at 212 length 22 bases, Tm 66
SSR size	218 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product; 51C anneal, product;
Sequence	WMC195
Source Species	Triticum aestivum