GrainGenes Marker Report: Xwmc201-6A
Locus
Xwmc201-6A
Type
Microsatellite
Chromosome
6A
Map
Conan/Reeder-6A 0 Kofa/UC1113-6A 67.1 Ta-SSR-2004-6A 46.4 Ta-Synthetic/Opata-GPW-6A 91.4
[ Show Nearby Loci ]
Map Data
Durum wheat, Kofa x UC1113 Wheat, Conan x Reeder Wheat, Consensus SSR, 2004 Wheat, Synthetic x Opata, GPW
Probe
WMC201
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc201 wmc201-6A wmc201
Probe for Locus
WMC201
Locus
Xwmc201-6A Xwmc201 wmc201-6A wmc201
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 4f12 Donor: Morell, Canberra Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 243 bases located on 6A Trimmed to insert at both ends (remving mostly Ns). (CA)25 201 to 250. Bases 162 to 249 show homology to 26f10 bases 402 to 489 (88 in longest run, maxScore = 114).
Type
PCR SSR
PCR primers
Forward cATgcTcTTTcAcTTgggTTcg at 85 length 22 bases, Tm 66
Reverse gcgcTTgcAggAATTcAAcAcT at 312 length 22 bases, Tm 67
SSR size
249 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence
WMC201
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc201-6A
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