Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Marker Report: Xwmc216.3eb

[Submit comment/correction]

Locus	Xwmc216.3eb
Type	Microsatellite
Chromosome	5A
Map	Wheat-Composite2004-5A 80
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004
Probe	WMC216
Possible Orthologs	wmc216-1D [ Show all 7 ]
Probe for Locus	WMC216
Locus	Xwmc216-1D [ Show all 8 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 11h7 Donor: Benoist Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 128 bases located on 1D AGR205.8. Trimed to insert at 5' end and to Rsa1 at 3' end. (GT)22 42 to 85.
Type	PCR SSR
PCR primers	Forward AcgTATccAgAcAcTgTggTAA at 13 length 22 bases, Tm 59 Reverse TAATggTggATccATgATAgcc at 114 length 22 bases, Tm 63
SSR size	123 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC216
Source Species	Triticum aestivum