GrainGenes Marker Report: Xwmc221-7D
Locus
Xwmc221-7D
Type
Microsatellite
Chromosome
7D
Map
CS/SQ1-7D 142.3 Ta-SSR-2004-7D 91.7
[ Show Nearby Loci ]
Map Data
Wheat, Chinese Spring x SQ1 Wheat, Consensus SSR, 2004
Probe
WMC221
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc221
Probe for Locus
WMC221
Locus
Xwmc221-7D Xwmc221
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 11g9 Donor: Benoist Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 213 bases located on 7D AGR205.23. Trimmed to Rsa1 at both ends. (CA)16 176 to 207, (CT)9 158 to 175.
Type
PCR SSR
PCR primers
Forward AcgATAATgcAgcggggAAT at 40 length 20 bases, Tm 65
Reverse gcTgggATcAAgggATcAAT at 229 length 20 bases, Tm 63
SSR size
209 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence
WMC221
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc221-7D
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