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GrainGenes Marker Report: Xwmc222-1D

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Locus	Xwmc222-1D
Type	Microsatellite
Chromosome	1D
Map	Conan/Reeder-1D 0 Ta-SSR-2004-1D 29.7 Ta-Synthetic/Opata-GPW-1D 36
	[ Show Nearby Loci ]
Map Data	Wheat, Conan x Reeder Wheat, Consensus SSR, 2004 Wheat, Synthetic x Opata, GPW
Probe	WMC222
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs	Xwmc222 Xwmc222-3B wmc222 wmc222-1D
Probe for Locus	WMC222
Locus	Xwmc222-1D Xwmc222-3B Xwmc222 wmc222 wmc222-1D
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 11b10 Donor: Benoist Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 148 bases located on 1D, 166 bases located on 1D AGR205.26. Trimmed to rsa1 at 5' end and to insert at 3' end. (GT)15 167 to 196. Bases 167 to 196 show homology to 17g10 bases 78 to 107 (30 in longest run, maxScore = 55).
Type	PCR SSR
PCR primers	Forward AAAggTgcgTTcATAgAAAATTAgA at 117 length 25 bases, Tm 61 Reverse AgAggTgTTTgAgAcTAATTTggTA at 255 length 25 bases, Tm 59
SSR size	163 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC222
Source Species	Triticum aestivum