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GrainGenes Marker Report: Xwmc223

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Locus	Xwmc223
Type	Microsatellite
Map	Ta-NxW-2B 120.6
	[ Show Nearby Loci ]
Map Data	Wheat, Nanda2419 x Wangshuibai
Probe	WMC223
Probe for Locus	WMC223
Locus	Xwmc223
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 11e10 Donor: Benoist AGR205.29. Trimmed to insert at both ends. (CA)15 214 to 243.
Type	PCR SSR
PCR primers	Forward cTcTcggTTgcAAAcAAccAcA at 133 length 22 bases, Tm 67 Reverse cAgggAcAAcAcAcgAcAAAgT at 254 length 22 bases, Tm 64
SSR size	143 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC223
Source Species	Triticum aestivum