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GrainGenes Marker Report: Xwmc230

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Locus	Xwmc230
Type	Microsatellite
Chromosome	1B
Map	Ta-NxW-1B 46.2 Wheat-Composite2004-1B 46
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Map Data	Wheat, Composite, 2004 Wheat, Nanda2419 x Wangshuibai
Probe	WMC230
Possible Orthologs	Xwmc230-1B wmc230 wmc230-1B
Probe for Locus	WMC230
Locus	Xwmc230-1B Xwmc230 wmc230 wmc230-1B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 11b12 Donor: Benoist AGR205.42. Apparently no overlap between forward and reverse sequences. Trimmed to Rsa1 at 5' end and to insert at 3' end. Multiple inserts in this clone joined at 345-369 by linkers. (GT)14 92 to 119. Bases 375-445 match part of 3f10. Bases 459 to 481 show homology to 24c3 bases 274 to 296 (23 in longest run, maxScore = 69).
Type	PCR SSR
PCR primers	Forward AgAAgcgAgcAggTgTgTTTgA at 6 length 22 bases, Tm 66 Reverse cTgcTTccTcccAcAAcAgATg at 195 length 22 bases, Tm 66
SSR size	213 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence	WMC230
Source Species	Triticum aestivum