GrainGenes Marker Report: Xwmc231-3B
Locus
Xwmc231-3B
Type
Microsatellite
Chromosome
3B
Map
CS/SQ1-3B 76.5 Ta-SSR-2004-3B 55.9
[ Show Nearby Loci ]
QTL with Locus Marker
QMat.crc-3B
Map Data
Wheat, Chinese Spring x SQ1 Wheat, Consensus SSR, 2004
Probe
WMC231
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
wmc231 Xwmc231
Probe for Locus
WMC231
Locus
Xwmc231-3B Xwmc231 wmc231
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 11c12 Donor: Benoist Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 140 bases located on 3B, 228 bases located on 3B AGR205.43. Trimmed to Rsa1 at 5' end and to insert at 3' end. (GA)10 127 to 146, (GT)8 81 to 96.
Type
PCR SSR
PCR primers
Forward cATggcgAggAgcTcggTggTc at 8 length 22 bases, Tm 74
Reverse gTggAgcAcAggcggAgcAAgg at 215 length 22 bases, Tm 74
SSR size
229 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence
WMC231
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc231-3B
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