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GrainGenes Marker Report: Xwmc232-4A

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Locus
Xwmc232-4A
Type
Microsatellite
Chromosome
4A
Chromosome Arm
4AL
Map
CS/SQ1-4A52.9
Ta-SSR-2004-4A68.1
Ta-Synthetic/Opata-GPW-4A110.3
Ta-Synthetic/Opata-SSR2-4A124.4
[ Show Nearby Loci ]
Map Data
Durum wheat, Svevo x Ciccio
Wheat, Chinese Spring x SQ1
Wheat, Consensus SSR, 2004
Wheat, Synthetic x Opata, GPW
Wheat, Synthetic x Opata, SSR 2
Probe
WMC232
Reference
ReferenceSomers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc232
Xwmc232-7B
Xwmc232.2
wmc232
wmc232-4A



Probe for Locus
WMC232
Locus
Xwmc232-4A
[ Show all 6 ]
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 5b1.1
Donor: Sharp, Camden
Trimmed to Rsa1 at both ends. Multiple inserts (2) joined by linker related sequence 310-317.(CA)11 367 to 388, (CA)14 391 to 418, (GT)23 56 to 101. Primers shown are for first insert.
Type
PCR
SSR
PCR primers
Forward gAgATTTgTTcATTTcATcTTcgcA at 23 length 25 bases, Tm 65
Reverse TATATTAAAggTTAgAggTAgTcAg at 106 length 25 bases, Tm 52
SSR size
108 bases expected, Chinese Spring
Amplification Conditions
51C anneal, product;
Sequence
WMC232
Source Species
Triticum aestivum