GrainGenes Marker Report: Xwmc233-5D
Locus
Xwmc233-5D
Type
Microsatellite
Chromosome
5D
Chromosome Arm
5DS
Map
CS/SQ1-5D 18.4 Ta-SSR-2004-5D 0 Ta-Synthetic/Opata-GPW-5D -5.9 Ta-Synthetic/Opata-SSR2-5D 0
[ Show Nearby Loci ]
Map Data
Wheat, Chinese Spring x SQ1 Wheat, Consensus SSR, 2004 Wheat, Synthetic x Opata, GPW Wheat, Synthetic x Opata, SSR 2
Probe
WMC233
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc233 wmc233
Probe for Locus
WMC233
Locus
Xwmc233-5D Xwmc233 wmc233
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 5b1.2 Donor: Sharp, Camden Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 262 bases located on 5D Trimmed to Rsa1 at both ends. Multiple inserts (2) joined by linker related sequence 310-317.(CA)11 367 to 388, (CA)14 391 to 418, (GT)23 56 to 101. Primers shown are for second insert.
Type
PCR SSR
PCR primers
Forward gAcgTcAAgAATcTTcgTcggA at 318 length 22 bases, Tm 66
Reverse ATcTgcTgAgcAgATcgTggTT at 556 length 22 bases, Tm 65
SSR size
260 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product;
Sequence
WMC233
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc233-5D
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