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GrainGenes Marker Report: Xwmc235-5B

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Locus	Xwmc235-5B
Type	Microsatellite
Chromosome	5B
Map	Ta-SSR-2004-5B 138.7
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Map Data	Durum wheat, Svevo x Ciccio Wheat, Consensus SSR, 2004
Probe	WMC235
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs	Xwmc235 wmc235-5B wmc235
Probe for Locus	WMC235
Locus	Xwmc235 Xwmc235-5B wmc235-5B wmc235
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 5d1 Donor: Sharp, Camden Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 231 bases located on 5B Trimmed to insert at 5' end and to Rsa1 at 3' end.(CA)25 178 to 227
Type	PCR SSR
PCR primers	Forward AcTgTTccTATccgTgcAcTgg at 86 length 22 bases, Tm 65 Reverse gAggcAAAgTTcTggAggTcTg at 299 length 22 bases, Tm 64
SSR size	235 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC235
Source Species	Triticum aestivum