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GrainGenes Marker Report: Xwmc237

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Locus
Xwmc237
Type
Microsatellite
Synonym
Other nameXAWM237
Chromosome
1A
Map
Wheat-Composite2004-1A115
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Map Data
Wheat, Composite, 2004
Probe
WMC237



Probe for Locus
WMC237
Locus
Xwmc237
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 5e2
Donor: Sharp, Camden
Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 130 bases located on 7B, 90 bases located on 7B
Trimmed to Rsa1 at 5' end and to insert at 3' end. (TG)6(TC)1(TG)6(TT)1(TG)6 at 119-158.
Type
PCR
SSR
PCR primers
Forward TAAgTgAATcgATgggcgTggA at 97 length 22 bases, Tm 68
Reverse cAgTgTgcAggggAAgcAgTgT at 200 length 22 bases, Tm 69
SSR size
125 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product;
Sequence
WMC237
Source Species
Triticum aestivum