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GrainGenes Marker Report: Xwmc24-1A

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Locus	Xwmc24-1A
Type	Microsatellite
Chromosome	1A
Chromosome Arm	1AS
Map	Kofa/UC1113-1A 39.1 Louise/Penawawa-1A 43.3 Ta-SSR-2004-1A 48.4 Ta-Synthetic/Opata-GPW-1A 35.3 Ta-Synthetic/Opata-SSR2-1A 38.7
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Map Data	Durum wheat, Kofa x UC1113 Wheat, Consensus SSR, 2004 Wheat, Louise x Penawawa Wheat, Synthetic x Opata, GPW Wheat, Synthetic x Opata, SSR 2
Probe	WMC24
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs	Xwmc24 wmc24 wmc24-1A
Probe for Locus	WMC24
Locus	Xwmc24-1A Xwmc24 wmc24 wmc24-1A
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 2b9 Donor: Wang, Utah Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 136-155 bases located on 1A (GT)28 25 to 80.
Type	PCR SSR
PCR primers	Forward gTgAgcAATTTTgATTATAcTg at 4 length 22 bases, Tm 54 Reverse TAcccTgATgcTgTAATATgTg at 134 length 22 bases, Tm 57
SSR size	152 bases expected, Chinese Spring
Amplification Conditions	51C anneal, polymorphic product on agarose; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC24
Source Species	Triticum aestivum