GrainGenes Marker Report: Xwmc243a-2B
Locus
Xwmc243a-2B
Type
Microsatellite
Map
CS/SQ1-2B 28.2
[ Show Nearby Loci ]
Map Data
Wheat, Chinese Spring x SQ1
Probe
WMC243
Possible Orthologs
Xwmc243
[ Show all 11 ]
Probe for Locus
WMC243
Locus
Xwmc243a-2B
[ Show all 12 ]
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 5e4 Donor: Sharp, Camden Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 158 bases located on 7D, 165 bases located on 2B Trimmed to Rsa1 at 5' end and to insert at 3' end. (CA)15 39 to 68. Small common sequence with 11D6 avoided.
Type
PCR SSR
PCR primers
Forward cgTcATTTccTcAAAcAcAccT at 8 length 22 bases, Tm 63
Reverse AccggcAgATgTTgAcAATAgT at 163 length 22 bases, Tm 62
SSR size
177 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence
WMC243
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc243a-2B
|
| |||||
|
| |||||
|
| |||||
| ||||||
|
| |||||
|
| |||||
|
| |||||
|
| |||||
|
| |||||
|
| |||||
|
| |||||
|
| |||||
|
| |||||
|
| |||||
|
| |||||
|
| |||||
|
|