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GrainGenes Marker Report: Xwmc247-5B

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Locus
Xwmc247-5B
Type
Microsatellite
Map
Ta-Synthetic/Opata-GPW-5B24.3
[ Show Nearby Loci ]
Map Data
Wheat, Synthetic x Opata, GPW
Probe
WMC247
Possible Orthologs
Xwmc247



Probe for Locus
WMC247
Locus
Xwmc247-5B
Xwmc247
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 5d5.2
Donor: Sharp, Camden
Derived from full sequence - this is the second insert only, without linkers. Primers shown are for this insert insert. (GT)13 107-132.
Type
PCR
SSR
PCR primers
Forward cATgTTgATAAccgAcTcgcAg at 80 length 22 bases, Tm 65
Reverse TgAAAcTAggggccAcgAATAA at 137 length 22 bases, Tm 64
SSR size
79 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence
WMC247
Source Species
Triticum aestivum