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GrainGenes Marker Report: Xwmc25-2B

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Locus
Xwmc25-2B
Type
Microsatellite
Chromosome
2B
Chromosome Arm
2BS
Map
P91193D1/P92201D5-2B88.2
Ta-SSR-2004-2B25
Ta-Synthetic/Opata-GPW-2B8.7
Ta-Synthetic/Opata-SSR2-2B38.7
[ Show Nearby Loci ]
Map Data
Wheat, Consensus SSR, 2004
Wheat, P91193D1 x P92201D5
Wheat, Synthetic x Opata, GPW
Wheat, Synthetic x Opata, SSR 2
Probe
WMC25
Reference
ReferenceSomers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc25-2D
[ Show all 18 ]



Probe for Locus
WMC25
Locus
Xwmc25b-2B
[ Show all 19 ]
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 2c9
Donor: Wang, Utah
(GT)26 193 to 244. >2 Polymorphic loci.
Type
PCR
SSR
PCR primers
Forward TcTggccAggATcAATATTAcT at 102 length 22 bases, Tm 59.0
Reverse TAAgATAcATAgATccAAcAcc at 246 length 22 bases, Tm 53.0
SSR size
166 bases expected, Chinese Spring
Amplification Conditions
51C anneal, polymorphic product on agarose; 51C anneal, polymorphic on acrylamide.;
Sequence
WMC25
Source Species
Triticum aestivum