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GrainGenes Marker Report: Xwmc254-1A

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Locus	Xwmc254-1A
Type	Microsatellite
Chromosome	1A
Chromosome Arm	1AL
Map	Ta-Synthetic/Opata-GPW-1A 152.2 Ta-Synthetic/Opata-SSR2-1A 136
	[ Show Nearby Loci ]
Map Data	Durum wheat, Svevo x Ciccio Wheat, Synthetic x Opata, GPW Wheat, Synthetic x Opata, SSR 2
Probe	WMC254
Possible Orthologs	Xwmc254 [ Show all 7 ]
Probe for Locus	WMC254
Locus	Xwmc254-1A [ Show all 8 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 4b2 Donor: Gupta, Meerut No trimming done to sequence. (CA)28 31 to 86. Forward primer is not ideal.
Type	PCR SSR
PCR primers	Forward AgTAATcTggTccTcTcTTcTTcT at 2 length 24 bases, Tm 58 Reverse AggTAATcTccgAgTgcAcTTcAT at 171 length 24 bases, Tm 64
SSR size	193 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC254
Source Species	Triticum aestivum