GrainGenes Marker Report: Xwmc258-4A
Locus
Xwmc258-4A
Type
Microsatellite
Chromosome
4A
Map
CS/SQ1-4A 91 Kofa/UC1113-4A 57.8 P91193D1/P92201D5-4A 67.3 Ta-SSR-2004-4A 38.9 Ta-Synthetic/Opata-GPW-4A 67.5
[ Show Nearby Loci ]
Map Data
Durum wheat, Kofa x UC1113 Wheat, Chinese Spring x SQ1 Wheat, Consensus SSR, 2004 Wheat, P91193D1 x P92201D5 Wheat, Synthetic x Opata, GPW
Probe
WMC258
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc258 Xwmc258-5B wmc258 wmc258-4A wmc258-5B
Probe for Locus
WMC258
Locus
Xwmc258-4A
[ Show all 6 ]
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 4f3 Donor: Gupta, Meerut Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 275 bases located on 4A No trimming done to sequence. (GT)26 115 to 166. Bases 251 to 270 show homology to 17c8 bases 101 to 120 (20 in longest run, maxScore = 43). Bases 112 to 166 show homology to 20b7 bases 241 to 295 (55 in longest run, maxScore = 61).
Type
PCR SSR
PCR primers
Forward gcgATgTcAgATATccgAAAgg at 75 length 22 bases, Tm 65
Reverse AccAggAcAccAgAAcAgcAAT at 346 length 22 bases, Tm 65
SSR size
293 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence
WMC258
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc258-4A
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