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GrainGenes Marker Report: Xwmc261-7B

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Locus
Xwmc261-7B
Type
Microsatellite
Map Data
Wheat, Consensus SSR, 2004
Probe
WMC261
Possible Orthologs
wmc261
[ Show all 12 ]



Probe for Locus
WMC261
Locus
Xwmc261-2A
[ Show all 13 ]
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 4e5
Donor: Gupta, Meerut
Trimmed to Rsa1 at 5' end and to insert at 3' end. (GT)12 68 to 91.
Type
PCR
SSR
PCR primers
Forward gATgTgcATgTgAATcTcAAAAgTA at 37 length 25 bases, Tm 62
Reverse AAAgAgggTcAcAgAATAAccTAAA at 122 length 25 bases, Tm 60
SSR size
110 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product;
Sequence
WMC261
Source Species
Triticum aestivum