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GrainGenes Marker Report: Xwmc262

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Locus	Xwmc262
Type	Microsatellite
Chromosome	4A
Chromosome Arm	4AL
Map	Tt-LxG-4A 94.6 Wheat, UC1110 x PI610750 4A 112.2 Wheat-Composite2004-4A 114
	[ Show Nearby Loci ]
Map Data	T. turgidum, Langdon x G18-16 Wheat, Composite, 2004 Wheat, UC1110 x PI610750
Probe	WMC262
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	Xwmc262-4A wmc262 wmc262-4A
Probe for Locus	WMC262
Locus	Xwmc262-4A Xwmc262 wmc262 wmc262-4A
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 4g5 Donor: Gupta, Meerut Trimmed to insert at 5' end and to Rsa1 at 3' end. (GA)29 86 to 143.
Type	PCR SSR
PCR primers	Forward gcTTTAAcAAAgATccAAgTggcAT at 9 length 25 bases, Tm 65 Reverse gTAAAcATccAAAcAAAgTcgAAcg at 182 length 25 bases, Tm 64
SSR size	198 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence	WMC262
Source Species	Triticum aestivum