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GrainGenes Marker Report: Xwmc262-4A

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Locus	Xwmc262-4A
Type	Microsatellite
Chromosome	4A
Chromosome Arm	4AL
Map	CS/SQ1-4A 79.7 Kofa/UC1113-4A 103.1 Ta-SSR-2004-4A 60.9 Ta-Synthetic/Opata-GPW-4A 108.8 Ta-Synthetic/Opata-SSR2-4A 120.3
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Map Data	Durum wheat, Kofa x UC1113 Wheat, Chinese Spring x SQ1 Wheat, Consensus SSR, 2004 Wheat, Synthetic x Opata, GPW Wheat, Synthetic x Opata, SSR 2
Probe	WMC262
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs	Xwmc262 wmc262 wmc262-4A
Probe for Locus	WMC262
Locus	Xwmc262-4A Xwmc262 wmc262 wmc262-4A
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 4g5 Donor: Gupta, Meerut Trimmed to insert at 5' end and to Rsa1 at 3' end. (GA)29 86 to 143.
Type	PCR SSR
PCR primers	Forward gcTTTAAcAAAgATccAAgTggcAT at 9 length 25 bases, Tm 65 Reverse gTAAAcATccAAAcAAAgTcgAAcg at 182 length 25 bases, Tm 64
SSR size	198 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence	WMC262
Source Species	Triticum aestivum