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GrainGenes Marker Report: Xwmc265-2B

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Locus	Xwmc265-2B
Type	Microsatellite
Chromosome	2B
Chromosome Arm	2BS
Map	Ta-SSR-2004-2B 61.7 Ta-Synthetic/Opata-GPW-2B 62.2 Ta-Synthetic/Opata-SSR2-2B 109.6
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Map Data	Wheat, Consensus SSR, 2004 Wheat, Synthetic x Opata, GPW Wheat, Synthetic x Opata, SSR 2
Probe	WMC265
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs	Xwmc265 wmc265 wmc265-2B wmc265-6B
Probe for Locus	WMC265
Locus	Xwmc265-2B Xwmc265 wmc265 wmc265-2B wmc265-6B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 4d6 Donor: Gupta, Meerut Trimmed to insert at 3' end. (GT)18 151 to 186. Bases 47-167 (ie. 5' side of microsatellite) match part of 1B3.
Type	PCR SSR
PCR primers	Forward gTggATAAcATcATggTcAAc at 15 length 21 bases, Tm 57 Reverse TAcTTcgcAcTAgATgAgccT at 257 length 22 bases, Tm 59
SSR size	263 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC265
Source Species	Triticum aestivum