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GrainGenes Marker Report: Xwmc272-2B

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Locus	Xwmc272-2B
Type	Microsatellite
Chromosome	2B
Chromosome Arm	2BS
Map	Ta-SSR-2004-2B 56.6 Ta-Synthetic/Opata-GPW-2B 59.3 Ta-Synthetic/Opata-SSR2-2B 106.6
	[ Show Nearby Loci ]
Map Data	Wheat, Consensus SSR, 2004 Wheat, Synthetic x Opata, GPW Wheat, Synthetic x Opata, SSR 2
Probe	WMC272
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs	Xwmc272 wmc272 wmc272-2B
Probe for Locus	WMC272
Locus	Xwmc272-2B Xwmc272 wmc272 wmc272-2B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 3e8 Donor: Keller, Zurich No trimming done to sequence. May be multple inserts (2) joined at 433-442 by linker. First insert is a (near) duplicate of 9G1.(GA)9 340 to 357, (GT)8 288 to 303, (GT)9 318 to 335, (GT)26 508 to 559. Primers here are for second insert. Use reverse primer at close to Tm (risk of secondary priming). Bases 98-192 match part of 3e12 (complement).
Type	PCR SSR
PCR primers	Forward TcAggccATgTATTATgcAgTA at 452 length 22 bases, Tm 60 Reverse AcgAccAggATAgccAATTcAA at 570 length 22 bases, Tm 65
SSR size	140 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC272
Source Species	Triticum aestivum