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GrainGenes Marker Report: Xwmc283.2-4A

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Locus
Xwmc283.2-4A
Type
Microsatellite
Map
CS/SQ1-4A75.4
[ Show Nearby Loci ]
Map Data
Wheat, Chinese Spring x SQ1
Probe
WMC283
Possible Orthologs
Xwmc283
[ Show all 17 ]



Probe for Locus
WMC283
Locus
Xwmc283.2-4A
[ Show all 17 ]
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 13d1
Donor: Jager-Gussen, Saaten Union
Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 157 bases located on 7A
No sequence 14.7.97. Resequenced. Trimmed to insert at both ends. (CA)19 64 to 101, (CA)8 104 to 119.
Type
PCR
SSR
PCR primers
Forward cgTTggcTgggTTATATcATcT at 2 length 22 bases, Tm 62
Reverse gAcccgcgTgTAAgTgATAggA at 131 length 22 bases, Tm 65
SSR size
151 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence
WMC283
Source Species
Triticum aestivum