Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Marker Report: Xwmc285

[Submit comment/correction]

Locus	Xwmc285
Type	Microsatellite
Chromosome	4D
Map	Wheat, UC1110 x PI610750 4D 0 Wheat-Composite2004-4D 0
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004 Wheat, UC1110 x PI610750
Probe	WMC285
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	Xwmc285-4D wmc285
Probe for Locus	WMC285
Locus	Xwmc285 Xwmc285-4D wmc285
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 13f1.1 Donor: Jager-Gussen, Saaten Union Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 298 bases located on 4D Trimmed to insert at 3' end, 5' end not located. Multiple inserts (2?) joined 371-380 by single linker - this is first insert . (CA)10 18 to 37, (CA)20 73 to 112.
Type	PCR SSR
PCR primers	Forward TgTggTTgTATTTgcggTATgg at 42 length 22 bases, Tm 64 Reverse TTgTggTgcTgAgTTAgcTTgT at 309 length 22 bases, Tm 62
SSR size	289 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product.
Sequence	WMC285
Source Species	Triticum aestivum