GrainGenes Marker Report: Xwmc285-4D
Locus
Xwmc285-4D
Type
Microsatellite
Chromosome
4D
Map
Ta-SSR-2004-4D 9.9
[ Show Nearby Loci ]
Map Data
Wheat, Consensus SSR, 2004
Probe
WMC285
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc285 wmc285
Probe for Locus
WMC285
Locus
Xwmc285 Xwmc285-4D wmc285
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 13f1.1 Donor: Jager-Gussen, Saaten Union Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 298 bases located on 4D Trimmed to insert at 3' end, 5' end not located. Multiple inserts (2?) joined 371-380 by single linker - this is first insert . (CA)10 18 to 37, (CA)20 73 to 112.
Type
PCR SSR
PCR primers
Forward TgTggTTgTATTTgcggTATgg at 42 length 22 bases, Tm 64
Reverse TTgTggTgcTgAgTTAgcTTgT at 309 length 22 bases, Tm 62
SSR size
289 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product.
Sequence
WMC285
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc285-4D
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