GrainGenes Marker Report: Xwmc286-7A
Locus
Xwmc286-7A
Type
Microsatellite
Map
P91193D1/P92201D5-7A 246.7
[ Show Nearby Loci ]
Map Data
Wheat, P91193D1 x P92201D5
Probe
WMC286
Possible Orthologs
Xwmc286.2 Xwmc286.4 Xwmc286.5
Probe for Locus
WMC286
Locus
Xwmc286-7A Xwmc286.5 Xwmc286.2 Xwmc286.4
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 13f1.2 Donor: Jager-Gussen, Saaten Union Trimmed to insert at both ends. Multiple inserts were joined 371-380 by single linker - this is second insert. (CA)9 328 to 345, (GA)14 346 to 373. Weak homology with 1b11.
Type
PCR SSR
PCR primers
Forward cgggTTcTcTggATcggTTc at 211 length 20 bases, Tm 66
Reverse ccccggccTTccTcATTcTT at 373 length 20 bases, Tm 68
SSR size
182 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product; 61C anneal, polymorphic on acrylamide (several loci).
Sequence
WMC286
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc286-7A
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