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GrainGenes Marker Report: Xwmc286.5

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Locus	Xwmc286.5
Type	Microsatellite
Chromosome	1A
Map	Wheat-Composite2004-1A 45
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004
Probe	WMC286
Possible Orthologs	Xwmc286-7A Xwmc286.2 Xwmc286.4
Probe for Locus	WMC286
Locus	Xwmc286-7A Xwmc286.5 Xwmc286.2 Xwmc286.4
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 13f1.2 Donor: Jager-Gussen, Saaten Union Trimmed to insert at both ends. Multiple inserts were joined 371-380 by single linker - this is second insert. (CA)9 328 to 345, (GA)14 346 to 373. Weak homology with 1b11.
Type	PCR SSR
PCR primers	Forward cgggTTcTcTggATcggTTc at 211 length 20 bases, Tm 66 Reverse ccccggccTTccTcATTcTT at 373 length 20 bases, Tm 68
SSR size	182 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide (several loci).
Sequence	WMC286
Source Species	Triticum aestivum