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GrainGenes Marker Report: Xwmc291

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Locus	Xwmc291
Type	Microsatellite
Chromosome	3B
Map	CIMMYT-integrated-3B 65.18 Ta-NxW-3B 162.2 Wheat-Composite2004-3B 55
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Map Data	Wheat, CIMMYT Integrated Wheat, Composite, 2004 Wheat, Nanda2419 x Wangshuibai
Probe	WMC291
Possible Orthologs	wmc291 Xwmc291-3B
Probe for Locus	WMC291
Locus	Xwmc291 Xwmc291-3B wmc291
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 13f2 Donor: Jager-Gussen, Saaten Union Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 239 bases located on 3B Trimmed to insert at 5' end and to insert at 3' end. (GT)26 174 to 225.
Type	PCR SSR
PCR primers	Forward TAccAcgggAAAggAAAcATcT at 26 length 22 bases, Tm 63 Reverse cAcgTTgAAAcAcggTgAcTAT at 237 length 22 bases, Tm 62
SSR size	233 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide (several loci).
Sequence	WMC291
Source Species	Triticum aestivum