GrainGenes Marker Report: Xwmc307-3B
Locus
Xwmc307-3B
Type
Microsatellite
Chromosome
3B
Map
Ta-SSR-2004-3B 65.4
[ Show Nearby Loci ]
Map Data
Wheat, Consensus SSR, 2004
Probe
WMC307
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
wmc307 wmc307-3B
Probe for Locus
WMC307
Locus
Xwmc307-3B wmc307-3B wmc307
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 8c5 Donor: Rouse, Novartis Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 146 bases located on 3B Contains (GT)8(GA)13. Trimed to Rsa1 at 5' end and to insert at 3 ' end. (GA)13 171 to 196, (GT)8 155 to 170.
Type
PCR SSR
PCR primers
Forward gTTTgAAgAccAAgcTccTccT at 81 length 22 bases, Tm 63
Reverse AccATAAccTcTcAAgAAcccA at 204 length 22 bases, Tm 62
SSR size
145 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide.
Sequence
WMC307
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc307-3B
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