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GrainGenes Marker Report: Xwmc31

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Locus	Xwmc31
Type	Microsatellite
Map	Ta-NxW-1B 70.3
	[ Show Nearby Loci ]
Map Data	Wheat, Nanda2419 x Wangshuibai
Probe	WMC31
Possible Orthologs	Xwmc31-1B wmc31 wmc31-1B
Probe for Locus	WMC31
Locus	Xwmc31 Xwmc31-1B wmc31 wmc31-1B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 2g10 Donor: Wang, Utah Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 130 bases located on 1B, 140 bases located on 1D CT CA compund microsatellite(GA)11 182 to 203, (GT)19 144 to 181. PCR product after PCR anneal 61C, polymorphic on agarose? Bases 150-279 (ie 3' side of microsatellite) homologous to part of 7c10.
Type	PCR SSR
PCR primers	Forward gTTcAcAcggTgATgAcTcccA at 122 length 22 bases, Tm 67 Reverse cTgTTgcTTgcTcTgcAcccTT at 238 length 22 bases, Tm 68
SSR size	138 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product;
Sequence	WMC31
Source Species	Triticum aestivum