GrainGenes Marker Report: Xwmc31-1B
Locus
Xwmc31-1B
Type
Microsatellite
Chromosome
1B
Map
Ta-SSR-2004-1B 29.9
[ Show Nearby Loci ]
Map Data
Wheat, Consensus SSR, 2004
Probe
WMC31
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc31 wmc31 wmc31-1B
Probe for Locus
WMC31
Locus
Xwmc31 Xwmc31-1B wmc31 wmc31-1B
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 2g10 Donor: Wang, Utah Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 130 bases located on 1B, 140 bases located on 1D CT CA compund microsatellite(GA)11 182 to 203, (GT)19 144 to 181. PCR product after PCR anneal 61C, polymorphic on agarose? Bases 150-279 (ie 3' side of microsatellite) homologous to part of 7c10.
Type
PCR SSR
PCR primers
Forward gTTcAcAcggTgATgAcTcccA at 122 length 22 bases, Tm 67
Reverse cTgTTgcTTgcTcTgcAcccTT at 238 length 22 bases, Tm 68
SSR size
138 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product;
Sequence
WMC31
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc31-1B
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