GrainGenes Marker Report: Xwmc310-4B
Locus
Xwmc310-4B
Type
Microsatellite
Chromosome
4B
Map
Ta-SSR-2004-4B 37.3
[ Show Nearby Loci ]
Map Data
Wheat, Consensus SSR, 2004
Probe
WMC310
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc310 wmc310
Probe for Locus
WMC310
Locus
Xwmc310 Xwmc310-4B wmc310
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 14f1 Donor: Hjortsholm, Sejet Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 191 bases located on 4B Trimmed to insert at 5' end and to Rsa1 at 3' end. (GT)10 329 to 348 plus (GT)4.
Type
PCR SSR
PCR primers
Forward TgTgAggcTgggAggAAAAgAg at 205 length 22 bases, Tm 67
Reverse gcTAggTTgTgTcccAcAATgc at 376 length 22 bases, Tm 66
SSR size
193 bases expected, Chinese Spring
Amplification Conditions
tested 61C anneal, no product; 51C anneal, product; 51C anneal, polymorphic on acrylamide.
Sequence
WMC310
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc310-4B
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