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GrainGenes Marker Report: Xwmc312

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Locus	Xwmc312
Type	Microsatellite
Chromosome	1A
Map	Ta-NxW-1A 94.2 Wheat-Composite2004-1A 63
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004 Wheat, Nanda2419 x Wangshuibai
Probe	WMC312
Possible Orthologs	Xwmc312-1A wmc312 wmc312-1A
Probe for Locus	WMC312
Locus	Xwmc312-1A Xwmc312 wmc312 wmc312-1A
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 14b2 Donor: Hjortsholm, Sejet Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 226 bases located on 1A Trimmed to Rsa1 at 5' end, and to insert at 3' end. (GA)14 225 to 252, (GA)10 255 to 274.
Type	PCR SSR
PCR primers	Forward TgTgcccgcTggTgcgAAg at 77 length 19 bases, Tm 73 Reverse ccgAcgcAggTgAgcgAAg at 285 length 19 bases, Tm 70
SSR size	227 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide (several loci).
Sequence	WMC312
Source Species	Triticum aestivum