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GrainGenes Marker Report: Xwmc318

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Locus	Xwmc318
Type	Microsatellite
Chromosome	5D
Map	Wheat-Composite2004-5D 21
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004
Probe	WMC318
Possible Orthologs	Xwmc318-5D wmc318
Probe for Locus	WMC318
Locus	Xwmc318 Xwmc318-5D wmc318
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 14e5 Donor: Hjortsholm, Sejet Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 207 bases located on 5D Trimmed to insert at 5' ends and to Rsa1 at 3' end. Bases 57-149 match part of 1g5. Bases 84 to 97 show homology to 1G5 bases 89 to 102 (14 in longest run, maxScore = 27). Forward primer does not overlap this homology. (GT)11 115 to 136. Bases 80 to 93 show homology to 10d12 bases 359 to 372 (complement) (14 in longest run, maxScore = 28).
Type	PCR SSR
PCR primers	Forward cgTAAAATTAcggTgcATTgAT at 19 length 22 bases, Tm 60 Reverse gTggAcTTTTgTggTTTTTgAg at 199 length 22 bases, Tm 60
SSR size	202 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product.
Sequence	WMC318
Source Species	Triticum aestivum