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GrainGenes Marker Report: Xwmc320

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Locus	Xwmc320
Type	Microsatellite
Chromosome	1B
Map	Wheat-Composite2004-1B 55
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Map Data	Wheat, Composite, 2004
Probe	WMC320
Possible Orthologs	wmc320
Probe for Locus	WMC320
Locus	Xwmc320 wmc320
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 14a6 Donor: Hjortsholm, Sejet Trimmed to Rsa1 at 5' end and to insert at 3' end. (CA)12 24 to 47.
Type	PCR SSR
PCR primers	Forward cATgcTTTcAAgAccTAcgAcA at 4 length 22 bases, Tm 62 Reverse gcAAAcTTgcTcTgcTTgAcTc at 78 length 22 bases, Tm 63
SSR size	96 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide.
Sequence	WMC320
Source Species	Triticum aestivum