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GrainGenes Marker Report: Xwmc322

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Locus	Xwmc322
Type	Microsatellite
Chromosome	7B
Map	Ta-NxW-3A 176.5 Wheat, UC1110 x PI610750 3A 164 Wheat-Composite2004-7B 102
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004 Wheat, Nanda2419 x Wangshuibai Wheat, UC1110 x PI610750
Probe	WMC322
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	Xwmc322-3A Xwmc322-3B wmc322 wmc322-3A
Probe for Locus	WMC322
Locus	Xwmc322-3A Xwmc322 Xwmc322-3B wmc322 wmc322-3A
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 17b7 Donor: Khairallah, Mexico Trimmed to insert at both ends. (GT)24 40 to 87. Forward primer reduced size to lower Tm.
Type	PCR SSR
PCR primers	Forward cgccccAcTATgcTTTg at 12 length 17 bases, Tm 61 Reverse cccAgTccAgcTAgccTcc at 88 length 19 bases, Tm 64
SSR size	95 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide.
Sequence	WMC322
Source Species	Triticum aestivum