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GrainGenes Marker Report: Xwmc322-3A

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Locus	Xwmc322-3A
Type	Microsatellite
Map	Conan/Reeder-3A 135.263 CS/SQ1-3A 133.5 T.b.xT.m.-3A 222.6
	[ Show Nearby Loci ]
Map Data	T. boeoticum x monococcum Wheat, Chinese Spring x SQ1 Wheat, Conan x Reeder
Probe	WMC322
Possible Orthologs	Xwmc322 Xwmc322-3B wmc322 wmc322-3A
Probe for Locus	WMC322
Locus	Xwmc322-3A Xwmc322 Xwmc322-3B wmc322 wmc322-3A
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 17b7 Donor: Khairallah, Mexico Trimmed to insert at both ends. (GT)24 40 to 87. Forward primer reduced size to lower Tm.
Type	PCR SSR
PCR primers	Forward cgccccAcTATgcTTTg at 12 length 17 bases, Tm 61 Reverse cccAgTccAgcTAgccTcc at 88 length 19 bases, Tm 64
SSR size	95 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide.
Sequence	WMC322
Source Species	Triticum aestivum