GrainGenes Marker Report: Xwmc323-7B
Locus
Xwmc323-7B
Type
Microsatellite
Chromosome
7B
Map
Kofa/UC1113-7B 9.7 Ta-SSR-2004-7B 1.2
[ Show Nearby Loci ]
Map Data
Wheat, Consensus SSR, 2004
Probe
WMC323
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc323 Xwmc323-7A wmc323 wmc323-7B
Probe for Locus
WMC323
Locus
Xwmc323-7B Xwmc323 wmc323 wmc323-7B Xwmc323-7A
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 17e7 Donor: Khairallah, Mexico Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 160 bases located on 7B Trimmed to insert at both ends. Bases 273 to 293 show homology to 9h4 bases 81 to 101 (21 in longest run, maxScore = 21) = mostly tetranucleotide repeat. (CA)11 253 to 274, (CA)11 297 to 318, (CT)11 231 to 252.
Type
PCR SSR
PCR primers
Forward AcATgATTgTggAggATgAggg at 180 length 22 bases, Tm 65
Reverse TcAAgAggcAgAcATgTgTTcg at 319 length 22 bases, Tm 65
SSR size
161 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product; 61C anneal, polymorphic on acrylamide.
Sequence
WMC323
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc323-7B
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