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GrainGenes Marker Report: Xwmc324

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Locus	Xwmc324
Type	Microsatellite
Map	Ta-NxW-5D 0
	[ Show Nearby Loci ]
Map Data	Wheat, Nanda2419 x Wangshuibai
Probe	WMC324
Probe for Locus	WMC324
Locus	Xwmc324
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 17g7 Donor: Khairallah, Mexico Trimmed to insert at 5' end and to Ssp1 at 3' end. Bases 27 to 61 (complement) show homology to 6e4 bases 28 to 62 (35 in longest run, maxScore = 45), zone is 1-80. Primers do not overlap the region. (CA)13 106 to 131, (CA)14 134 to 161.
Type	PCR SSR
PCR primers	Forward AAcTAgggggcAcggcAA at 86 length 18 bases, Tm 66 Reverse gATgTgATcgcAgTcggc at 231 length 18 bases, Tm 64
SSR size	163 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide (several loci).
Sequence	WMC324
Source Species	Triticum aestivum