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GrainGenes Marker Report: Xwmc327

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Locus	Xwmc327
Type	Microsatellite
Chromosome	5A
Map	Ta-NxW-5A 151.1 Wheat-Composite2004-5A 66
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Map Data	Wheat, Composite, 2004 Wheat, Nanda2419 x Wangshuibai
Probe	WMC327
Possible Orthologs	Xwmc327-5A wmc327 wmc327-5A
Probe for Locus	WMC327
Locus	Xwmc327-5A Xwmc327 wmc327 wmc327-5A
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 17e9 Donor: Khairallah, Mexico Trimmed to insert at both ends. Bases 1 to 15 show homology to 1H3 bases 116 to 130 (15 in longest run, maxScore = 24), zone is 1-40. Primers are outside of this region. (GT)25 150 to 199.
Type	PCR SSR
PCR primers	Forward TgcggTAcAggcAAggcT at 75 length 18 bases, Tm 65 Reverse TAgAAcgcccTcgTcggA at 240 length 18 bases, Tm 65
SSR size	183 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide.
Sequence	WMC327
Source Species	Triticum aestivum