GrainGenes Marker Report: Xwmc327-5A
Locus
Xwmc327-5A
Type
Microsatellite
Chromosome
5A
Chromosome Arm
5AL
Map
CS/SQ1-5A 91.6 Louise/Penawawa-5A 0 Ta-Synthetic/Opata-SSR2-5A 113.2
[ Show Nearby Loci ]
Map Data
Wheat, Chinese Spring x SQ1 Wheat, Louise x Penawawa Wheat, Synthetic x Opata, SSR 2
Probe
WMC327
Possible Orthologs
Xwmc327 wmc327 wmc327-5A
Probe for Locus
WMC327
Locus
Xwmc327-5A Xwmc327 wmc327 wmc327-5A
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 17e9 Donor: Khairallah, Mexico Trimmed to insert at both ends. Bases 1 to 15 show homology to 1H3 bases 116 to 130 (15 in longest run, maxScore = 24), zone is 1-40. Primers are outside of this region. (GT)25 150 to 199.
Type
PCR SSR
PCR primers
Forward TgcggTAcAggcAAggcT at 75 length 18 bases, Tm 65
Reverse TAgAAcgcccTcgTcggA at 240 length 18 bases, Tm 65
SSR size
183 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide.
Sequence
WMC327
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc327-5A
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