GrainGenes Marker Report: Xwmc331-4D
Locus
Xwmc331-4D
Type
Microsatellite
Chromosome
4D
Chromosome Arm
4DL
Map
Conan/Reeder-4D 221 Louise/Penawawa-4D 27.6 P91193D1/P92201D5-4D 0 Ta-SSR-2004-4D 42.9 Ta-Synthetic/Opata-SSR2-4D 24.9
[ Show Nearby Loci ]
Map Data
Wheat, Conan x Reeder Wheat, Consensus SSR, 2004 Wheat, Louise x Penawawa Wheat, P91193D1 x P92201D5 Wheat, Synthetic x Opata, SSR 2
Probe
WMC331
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc331 wmc331
Probe for Locus
WMC331
Locus
Xwmc331-4D Xwmc331 Alt2 wmc331
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 17f11 Donor: Khairallah, Mexico Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 134 bases located on 4D Trimmed to insert at 5' end and to insert at 3' end. (CA)13 56 to 81, (CT)10 36 to 55. Software struggled to get these primers.
Type
PCR SSR
PCR primers
Forward ccTgTTgcATAcTTgAccTTTTT at 2 length 23 bases, Tm 61
Reverse ggAgTTcAATcTTTcATcAccAT at 107 length 23 bases, Tm 61
SSR size
128 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product; 61C anneal, polymorphic on acrylamide.
Sequence
WMC331
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc331-4D
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