GrainGenes Marker Report: Xwmc332-2B
Locus
Xwmc332-2B
Type
Microsatellite
Chromosome
2B
Map
Louise/Penawawa-2B 0 Ta-SSR-2004-2B 93.4
[ Show Nearby Loci ]
Map Data
Durum wheat, Svevo x Ciccio Wheat, Consensus SSR, 2004 Wheat, Louise x Penawawa
Probe
WMC332
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc332 wmc332-2B wmc332
Probe for Locus
WMC332
Locus
Xwmc332-2B
[ Show all 6 ]
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 17b12 Donor: Khairallah, Mexico Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 172 bases located on 2B Trimed to insert at both ends. (CT)12 136 to 159.
Type
PCR SSR
PCR primers
Forward cATTTAcAAAgcgcATgAAgcc at 51 length 22 bases, Tm 65
Reverse gAAAAcTTTgggAAcAAgAgcA at 198 length 22 bases, Tm 62
SSR size
169 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide (several loci).
Sequence
WMC332
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc332-2B
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