Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Marker Report: Xwmc333.2

[Submit comment/correction]

Locus	Xwmc333.2
Type	Microsatellite
Chromosome	5B
Map	Wheat-Composite2004-5B 28
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004
Probe	WMC333
Possible Orthologs	Xwmc333 Xwmc333-1A wmc333-1A
Probe for Locus	WMC333
Locus	Xwmc333-1A Xwmc333 Xwmc333.2 wmc333-1A
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 12e1 Donor: Weibull, Svaloff Weibull Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 170 bases located on 1A, 177 bases located on 6A Trimmed to Rsa1 at both ends. (GT)15 187 to 216.
Type	PCR SSR
PCR primers	Forward TcAAgcATAggTggcTTcgg at 73 length 20 bases, Tm 65 Reverse AcAgcAgccTTcAAgcgTTc at 227 length 20 bases, Tm 65
SSR size	174 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide (several loci).
Sequence	WMC333
Source Species	Triticum aestivum