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GrainGenes Marker Report: Xwmc334

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Locus	Xwmc334
Type	Microsatellite
Chromosome	3B
Map	CIMMYT-integrated-3B 58.89 Wheat-Composite2004-3B 45
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Map Data	Wheat, CIMMYT Integrated Wheat, Composite, 2004
Probe	WMC334
Possible Orthologs	wmc334
Probe for Locus	WMC334
Locus	Xwmc334 wmc334
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 12b3 Donor: Weibull, Svaloff Weibull Trimmed to insert at 5' end and to Rsa1 at 5' end. (CA)5G(CA)6 at 162 to 185.
Type	PCR SSR
PCR primers	Forward cTcgAAgcAggATgcATTTA at 89 length 20 bases, Tm 61 Reverse TcAAATTTTgggATAgcggg at 186 length 20 bases, Tm 64
SSR size	117 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC334
Source Species	Triticum aestivum